Türk Nöroşirürji Dergisi 1999 , Vol 9 , Num 3
FLOW CYTOMETRY, P53 PROTEIN AND EPIDERMAL GROWTH FACTOR RECEPTOR IMMUNOREACTIVITY IN ASTROCYTOMAS
Abdülkadir REİS1, Süleyman BAYKAL2, Kayhan KUZEYLI2, Yavuz TEKELİOĞLU3, Yavuz ÖZORAN1, Ercüment OVALI4, -Ümit ÇOBANOĞLU1, Ertuğrul ÇAKIR2
1KTÜ Tıp Fakültesi Patoloji, Anabilim Dalı, Trabzon
2KTÜ Tıp Fakültesi Nöroşirürji, Anabilim Dalı, Trabzon
3KTÜ Tıp Fakültesi Histoloji, Anabilim Dalı, Trabzon
4KTÜ Tıp Fakültesi İç Hastalıkları (Hematoloji-Onkoloji) Anabilim Dalı, Trabzon
In recent years studies about flow cytometric DNA analysis of astrocytomas revealed the correlation between aneuploidy and turn or grade. Also, p53 and EGFr gene mutation rates were found to be high in these clinically aggressive tumors. Our study included 35 cases of astrocytomas diagnosed in KTU Pathology Department between 1994-1998. The cases are divided into two subgroups according to WHO-1993 classification as low and high grade tumors. Flow cytometric DNA analysis and p53- EGFr immunoreactivity are studied from paraffin embedded tissues. The relation between p53 pozitivityDNA aneuploidy and high grade tumors was statistically significant (p<0.05 and p<0.001, respectively). In contrast, S-indices and EGFr positivity were not statistically related to grade of the tumors. Mutant p53 protein and aneuploidy studies may be helpfull in grading especially in small biopsy specimens. Also, flow cytometric DNA analysis seems to be useful for predicting survival and therapy of the cases. Anahtar Kelimeler : Astrocytoma, flow cytometry, immunohistochemistry