Türk Nöroşirürji Dergisi 2005 , Vol 15 , Num 1
A Microanatomical Evaluation of the Blood Brain Barrier and Endothelial Basal Laminae in Intracerebral Foetal Central Nervous System Grafts
Atilla AKBAY1, Nejat AKALAN1, Türkan ERBENGİ2, Aykut ERBENGİ1
1Hacettepe Üniversitesi Tıp Fakültesi Nöroşirürji Anabilim Dalı, Ankara
2İstanbul Üniversitesi Tıp Fakültesi Histoloji Anabilim Dalı, İstanbul
OBJECTIVE: Neural grafting or stem cell transplantation to the central nervous system has become popular in clinical and experimental studies. After the transplantation, restoration of the blood brain barrier is necessary for graft survival. In our study the function and microanatomic structure of the blood brain barrier were evaluated.

METHODS: Forty-six albino rats were utilized in this study. Cell suspension grafts prepared from rat fetuses were given into the corpus callosum of albino rats. Then rats were sacrificed on certain days and light microscopy and electron microscopic evaluations were performed.

RESULTS: Although endothelial tight junctions and HRP impermeability were seen at day 20, thickness of basal laminae did not reach the original level. Basal laminae thickness reached normal levels at day 30.

CONCLUSION: The results of this study demonstrated that basal laminae are morphologically the latest generating part of the blood brain barrier complex. Contribution of the basal laminae to the impermeability of the blood brain barrier is not clear at the moment. More studies are required to disclose the function of basal laminae. Anahtar Kelimeler : Blood brain barrier, basal laminae, cell suspension graft, neural grafting, tight junctions