Türk Nöroşirürji Dergisi 2004 , Vol 14 , Num 2
The Relationship of BCL-2 and Cyclin A Expression and Prognosis in Astrocytomas
Kenan KOÇ1, Çetin EVLİYAOĞLU1, Cengiz ERÇİN2, Konuralp İLBAY1, Savaş CEYLAN1
1Kocaeli Üniversitesi Tıp Fakültesi Beyin ve Sinir Cerrahisi Anabilim Dalı
2Kocaeli Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı
OBJECTİVE: Although the combined surgery, radiotherapy and chemotherapy treatments, high grade astrocytomas are still cause for major mortality and morbidity. In our study, concentrations of cyclin A and bcl-2 were measured in astrocytoma cases and morbidity effect were examined in high grade (grade III-IV) cases.

METHODS: In our study 28 supratentorial diffuse astrocytoma cases were included. Retrospective details were collected from the personal files. Cyclin Aand bcl-2 levels were measured with biotin streptavidin immuno-enzymatic antigen staining system from tissue paraffin blocks. Results were evaluated with Mann-Whitney U, Kruskal- Wallis and Spearman correlation tests.

RESULTS: Cyclin A concentrations were measured in all groups showed significant differences especially between grade II (3.4±1.7%), grade III (18.4±10.1%), grade IV (15.5±8.8%). Bcl-2 levels were measured as grade II (1.6±0.9%), grade III (1.8±0.8%), grade IV (2.2±0.6%). Postoperative survival for grade III were 85.6± 26.1 weeks and 43.1±12.2 weeks for grade IV astrocytomas.

CONCLUSION: In our study, grade II astrocytomas showed significant correlation between cyclin A and bcl-2 levels, but not for grade III and IV astrocytomas. Mean bcl-2 levels showed correlation with histological malignity. Anahtar Kelimeler : Astrocytoma, bcl-2, cyclin-A, immunohistochemistry, prognosis