Türk Nöroşirürji Dergisi 2001 , Vol 11 , Num 3
Necmettin TANRIÖVER1, Ziya AKAR1, Mustafa UZAN1, Ayhan BİLİR2, Haydar DURAK3, Reşit CANBEYLİ4, Cengiz KUDAY1
1İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Nöroşirürji Anabilim Dalı, İstanbul
2İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Histoloji ve Embriyoloji ABD, İstanbul
3İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Patoloji Anabilim Dalı, İstanbul
4Boğaziçi Üniversitesi, Psikobiyoloji Laboratuarı, İstanbul
Experimental animal models are needed to highlight molecular and cellular characteristics of glial tumors, since the etiology and molecular genetics have not been delineated in these neoplasms. Our aim was to demonstrate the formation of C6 rat glioma model in Wistar rats through histopathological and neuroradiological examinations, and also to evaluate clinical findings and surveys of these rats. We wished to delineate the optimal time period and data for magnetic resonance imaging (MRI) in this particular tumor model. C6 glioma cells were implanted with the aid of stereotactic guidance into the brain of 16 Wistar rats. Magnetic resonance images, through 1.5 tesla (T) MR system with kneepa coils, were collected from 9 rats, between days 7 to 9 to confirm tumor formation. All rat brains, inoculated with 1x106 C6 glioma cells, demonstrated malignant glioma formation in histopathological examinations. MR images revealed abnormal signal changes in right hemispheres of all the rats examined, and these findings were confirmed as malignant gliomas with hematoxylineeosine staining. Median survey of 16 rats in C6 glioma model in our study was similar to the literature (29 days). Distinct from the literature, optimal results for C6 rat glioma model in 1.5 T MRI were observed in kneepa coil and with a slice thickness of 3mm. In conclusion, MRI plays a major role in the confirmation of tumor formation and evaluation of tumor growth rate with its response to therapy in in vivo glioma models. Anahtar Kelimeler : C6 rat glioma model, experimental MRI