3,4İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Histoloji AD., İstanbul OBJECTIVE: In this study, the effects of tirofiban, which is a non-peptide platelet GPIIb\IIIa-receptor antagonist, on CA1 hippocampal neurons were investigated on the rats subjected to the transient forebrain ischemia.
MATERIAL AND METHODS: The transient forebrain ischemia was performed by the occlusion of bilateral carotis communis arteries. The animals in the treatment group were divided into three groups and were received doses of either 0.4 μg/kg/minute or 0.8 μg/kg/minute of tirifoban, at 30 minutes before the ischemia, at immediately after the ischemia and one hour post-ischemia. At the end of 7 days, the rats were sacrificed, and the effects of the ischemia and the efficacy of the treatment were investigated by counting of the intact neurons in the hippocampal CA1 region.
RESULTS: A 37% rate of protective influence was seen on the rats treated with a dose of 0.4 μg/kg/minute at 30 minutes before ischemia. The protection in the group that were treated with a dose of 0.8 μg/kg/minute tirofiban infusion was found as 46.7%. With the 0.4 μg/kg/minute dose of tirofiban treatment, no protective effect was noted whereas with the 0.8 μg/kg/minute dose of tirofiban treatment there was 32.2% recovery. No significant results were observed in the treatment doses that started 1 hour after the ischemia.
CONCLUSION: Our findings have suggested that tirofiban may be an efficient alternative agent in the treatment of acute cerebral ischemia when administrated at appropriate dose and time during the post-injury period.
Anahtar Kelimeler : Brain ischemia, Neuroprotection, Platelet aggregation inhibitors, Platelet glycoprotein GPIIb/IIIa complex, rats